Qibai Pingfei capsule induces apoptosis of pulmonary artery smooth muscle cells in hypoxic rats by regulating PI3K/AKT/mitochondrial signaling pathway

Purpose: To investigate the effect of Qibai Pingfei capsules (QBPF) medicated serum on apoptosis rat pulmonary artery smooth muscle cells (PASMC) in hypoxic rats, and to determine relationship between that PI3K/Akt/mitochondrial pathway.Methods: Rat PASMCs were isolated, cultured, optimal time concentration QBPF determined by CCK-8 method. Hypoxic rats treated with QBPF, + LY294002, or SC79. Apoptosis mitochondrial membrane potential assessed using Annexin VFITC/ PI, Hoechst 33258, Rho123 staining. The protein expression levels AKT, P-AKT, apoptosis-related proteins evaluated via western blot.Results: studies showed was 24 h, while 20 %. V-FITC/PI double staining 33258 assay revealed significantly promoted (p < 0.05). test results inhibited level rats' PASMCs, which enhanced PI3K inhibitor LY29002 AKT agonist SC79 Western blot reduced P-AKT Bcl-2, raised Bad, Bax, CytC, casepase-9 casepase-3, blocked No major changes seen groups.Conclusion: In blocks PI3K/AKT signaling pathway regulates activation downstream Bcl-2 family members, thus activating triggering PASMC death.